STUDY ON LIQUID CONDITIONS IN SHAKING FLASKS FOR LACCASE PRODUCTION BY A WHITE ROT FUNGUS
- VernacularTitle:层孔菌产漆酶的摇瓶最适培养条件研究
- Author:
Cong-Bao KANG
;
Jian ZHAO
;
Qing-Xin LI
;
Yin-Bo QU
;
Pei-Ji GAO
;
- Publication Type:Journal Article
- Keywords:
White rot fungus, Laccase, Orthogonal experiment
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
A white-rot fungi Rigidoporus sp.W-1 which could produce laccase was isolated. The fermentation conditions in shaking flasks were investigated. The optimal carbon source was wheat bran and (NH 4) 2SO 4 was the optimal nitrogen source. The components of the medium were optimized by orthogonal experiment. When W-1 was cultured under the optimum conditions, the activity of laccase could get to 7.1U/mL in 7 days.A great amount of crude laccase could be obtained by adding fresh medium to the 7 days old mycelium.
