CLONING OF NATTOKINASE GENE AND EXPRESSION IN E. COLI
- VernacularTitle:纳豆激酶基因在E.coli HB101中的初步表达研究
- Author:
Li-Xin LUO
;
Zhi-Li HUANG
;
Ru-De YANG
;
Jun-Jian LING
;
Shi-Zhong LIANG
;
- Publication Type:Journal Article
- Keywords:
Nattokinase gene, Cloning, Expression, Plasmid stability
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
In this study, nattokinase gene was amplified by PCR using bacillus subtilis chromosomal DNA as template and cloned into expressed vector pBV220. After transforming recombinant plasmid into E.coli HB101, the recombinant strain was yielded. It was proved that expression products was secretive and expression protein was 12% of total cell protein by SDS-PAGE. Optimum culture time and inducing time was determined as 6h and 5h respectively. The plasmid stability studies showed that recombinant plasmid has excellent segregational stability but the structural stability was not good in the host cell.
