SELECTION OF HIGH-YIELD CAROTENOID PRODUCING STRAIN BY LASER MUTAGENESIS OF PROTOPLAST OF PENICILLIUM SP. PT95
- VernacularTitle:激光诱变青霉PT95原生质体选育类胡萝卜素高产菌株
- Author:
Jian-Rong HAN
;
Xian-Fang DONG
;
- Publication Type:Journal Article
- Keywords:
Penicillium sp., Protoplast, Carotenoid, Laser mutagenesis
- From:
Microbiology
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
A mutant strain L05 was screened from its parent strain Penicillium sp. PT95 by laser irradiation of protoplast. When LOS strain was incubated in Czapek' s agar plates for 20 d, both the sclerotia biomass and carotenoid content accumulated in sclerotia increased significantly compared with that of PT95 strain, and the increase rate reached respectively 98.6% and 28.3% . The carotenoid yield of L05 strain reached 381ug/plate, which was 2.54 times higher than that of PT95. The character of both sclerotia and carotenoid high productivity remained stable after three times of subculture. No sectored colony appeared during subculture.
