THE RESEARCH OF FOUR DRUG RESISYANCE GENE IN MYCOBACTERIUM TUBERCULOSIS
- VernacularTitle:结核分枝杆菌临床株4种耐药基因的检测与分析
- Author:
Hong-Min LI
;
Xue-Quong WU
;
Jian-Qin LIANG
;
Li XIAO
;
Shu-Xin ZHANG
;
Hui-Xin HAN
;
- Publication Type:Journal Article
- Keywords:
M. tuberculosis drug-resistance, PCR-SSCP
- From:
Microbiology
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
objective To study the reationship beween drug-resistance gene mutation and drug-resistance level in M. tuberculosis. Methods 108 M. tuberculosis clinical isolated strains from sputum specimens were analyzed by PCR-SSCP and traditional drug susceptibility tests. Results the gene mutation rate of SM, RFP, INH and EMBresistance climical isolated strains was 78.5%, 68.2%, 70.5% and 48.6% respectively, and the mutation rate of SM, RFP, INH and EMB high concentration resistance isolated strains was 86.5% , 89% , 84% and 48.6% respectively, but 28.5 % , 16.6% and 7.1% was the mutation rate of low concentration resistance strains. Conclusion The gene mutation was in relation with drug resistance level of M. tube rculosis. The gene mutation rate was hiher in high concentration resistance isolated strains than in low concentration resistane isolated strains.
