STUDIES ON A NEW METHOD FOR COUNTING LIVING BACTERIAL CELL NUMBER
- VernacularTitle:一种快速的活菌计数新方法研究
- Author:
Hong-Gang WEI
;
Yuan-Guang LI
;
Jian LIU
;
Guo-Min SHENG
;
Ji-Min WU
;
- Publication Type:Journal Article
- Keywords:
MTT Colorimetric method, Bacterial cell counting
- From:
Microbiology
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
MTT Colorimetric method is usually applied for measuring the living animal cell number. By changing the reaction temperature and the reaction time as well as the colorimetric wavelength, the improved MTT colorimetric method was established to count the living bacterial cell number. This new method was used to measure the living cell concentration in the process for culturing bacteria PBW1. The results measured by the improved MIT colorimetric method and dilute plate method are similar. Compared with other methods including the dilute plate method, the improved MTT colorimetric method has many advantages such as accuracy, quickness.