PURIFICATION AND PROPERTIES OF FUMARASE FROM ASPERGILLUS WENTII
- VernacularTitle:温特曲霉延胡索酸酶的提纯及性质研究
- Author:
Teng ZHENG
;
Qiao-Qin SHI
;
Son-Gang WU
;
- Publication Type:Journal Article
- Keywords:
Aspergillus wentii, Fumarase, Purification, Properties.
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The fumarase from the culture of Aspergillus wentii F-871 was partially by means of precipitation with protamine sulphate, ammonium sulfate fractionation and column chromatography on Sephadex G-200, and then the concentrated enzyme solution was freeze-dried. The comparative enzyme activity of the fumarase was increased by 31.70 times and reached 24.6 U/mg, and the recovery was 36.64% . The optimal pH and temperature was 8.0 and 30℃, respectively. The pH stability of fumarase ranged 6.0 - 8.5, and more than 90% of the enzyme activity remained after incubated at 35℃ for 30 min.