ISOLATION OF AN ESTERASE PRODUCER TRICHOSPRONBRASSICAE AND ITS CATALYTIC PERFORMANCE IN KINETICRESOLUTION OF KETOPROFEN
- VernacularTitle:酮基布洛芬拆分用酯酶产生菌的筛选及其催化特性
- Author:
Duan SHEN
;
Jian-He XU
;
Peng-Ri GONG
;
You-Yan LIU
;
Hui-Yuan WU
;
- Publication Type:Journal Article
- Keywords:
Chiral drag, Ketoprofen, Optical resolution, Trichosporon brassicae, Esterase
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
A strain of yeast capable of hydrolyzing ethyl ester of racemic Ketoprofen with high enantioselectivity has been isolated from soil after two-step enrichment. The yeast was identified as Trichosporon brassicae. The process of growth and enzyme production was investigated. The catalytic performance of the resting cell of KET4 in kinetic resolution of Ketoprofen was also investigated. When the conversion of substrate reached 41% , enantiomeric excess of the (S) - Ketoprofen produced was 91 % , indicating a high enantiomeric ratio of 45.
