Inhibition of in vitro Translation of Esterase mRNA of Dipterex-Resistant Mosquito (Culex pipiens pallens) by Antisense Nucleic Acids
- VernacularTitle:反义核酸抑制抗敌百虫淡色库蚊扩增酯酶mRNA体外翻译的研究
- Author:
Huaimin ZHU
;
Yading ZOU
;
- Publication Type:Journal Article
- Keywords:
organophosphorus resistance, esterase, antisense nucleic acid, Culex pipiens pallens
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the inhibitory effect of antisense nucleic acid on the in vitro translation of esterase mRNA from dipterex resistant Culex pipiens pallens . Methods 18 mer nucleic acid was synthesized and complementary to the translation initiation site of mRNA of dipterex resistant mosquitoes. The ODNs were annealed to the corresponding mRNA molecules and they were added to rabbit reticulocyte cell free system. The translation products were analyzed by SDS PAGE. After fixing, the gel was exposed to X ray film by autoradiography for analysis of protein synthesis. Results Six ?mol/L of ODNs elicited a 50% reduction in specific protein expression , and 20 ?mol/L of ODNs inhibited the expression of esterase by 80%. The SDS PAGE showed that the band of reduced amounts of 65 kDa protein for resistant mosquito was almost the same as that for sensitive sample. Conclusion Antisense oligonucleic acids to the esterase mRNA of dipterex resistant mosquito could effectively inhibit its in vitro translation.
