Determination of T Lymphocytes and Trace Elements in Spleen from Rats Infected with Toxoplasma gondii
- VernacularTitle:弓形虫病大鼠T淋巴细胞和脾微量元素的测定
- Author:
Zhihui GENG
;
Yanqiu FANG
;
Li LIU
;
Yi SHI
;
Shuhong LI
;
- Publication Type:Journal Article
- Keywords:
Toxoplasma gondii, trace element, rat, acid ?-naphthyl acetate esterase, lymphocyte
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the level of five trace elements(Fe 2+ ?Cu 2+ ?Zn 2+ ?Ca 2+ ?Mg 2+ )in the spleen and changes of T lymphocyte and its subtype variations in peripheral blood from the rats infected with Toxoplasma gondii . Methods Twenty rats were randomly and equally divided into two groups: control group and experiment group. Each rat in the experiment group received an ip injection of 2 ml normal saline containing 1.5?10 6 tachyzoites of T. gondii . On the 64th day after injection of T.gondii , the changes in T lymphocytes (TL) and their subgroups, the helper T lymphocytes (Th) and the suppressor T lymphocytes(Ts) in the peripheral blood of the rats with T.gondii were determined by the assay of the lymphocytes labeled with intercellular acid ? naphthyl acetate esterase. All the rats were killed and the atomic absorption method were used for detecting the level of trace elements in the spleen tissue. Results The number of TL and Th in experiment group was significantly lower than that of control ( P
