CELL CHEMICAL CHARACTERISTICS AND 16S rDNA SEQUENCE ANALYSIS OF A BLUE-PIGMENT-PRODUCING STRAIN
- VernacularTitle:产蓝色素放线菌细胞化学组分及16SrDNA序列分析
- Author:
Heng-Lin CUI
;
Ling LU
;
Yi-Nan CHEN
;
Qing CHANG
;
Chuan-Cao DAI
;
- Publication Type:Journal Article
- Keywords:
Streptomyces,Natural blue pigment,16S rDNA sequence,Phylogenetic tree
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
An actinomycetes which produced soluble blue pigment was isolated from the soil sample in Nanjing,China.Based on its cell chemical characteristics and 16S rDNA sequence we found that its cell wall contained L-diaminopimelic acid and glycine,the whole cell hydrolysates contained glucose and ribose,whole cell contained fatty acid from C14 to C17 with 12-methyltetradecanoic(anteiso-15) and 14-methylpentadentadecanoic acid(iso-16) as the major components.The results shown that,it belongs to the genus Streptomyces.Phylogenetic tree of 16S rDNA sequences indicated that all strains were clustered into 9 branches.All strains that could produce blue pigment were clustered into 2 branches,they were S.coelicolor、S.cyaneus.The isolate closely related to Streptomyces indigocolor with a similarity of 99.4% fell into S.cyaneus branch.
