EFFECTS OF SUBSTRATE DISPERSION AND DISSOLUTION ON MICROBIAL ENZYMATIC CONVERSION OF STEROID
- VernacularTitle:底物的分散和溶解对甾体微生物酶反应的影响
- Author:
Kui YANG
;
Xiao-Jing LI
;
Xia FENG
;
Qiang YIN
;
Hai-Yan ZHAO
;
- Publication Type:Journal Article
- Keywords:
Steroid,Microbial conversion,Surfactant,Ultrasonic irradiation,Solubility
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
The dissolution of substrate is the key step in steroid microbial conversion.The formation of surfactant micelle with a hydrophobic center directly results in increased pseudo-water-solubilities of hydrophobic compounds such as steroids.Results showed increased water solubility of steroid with the increased surfactant concentrations.Compared to mono-surfactant PSE,less quantity of multiple surfactants PSE-MGE was required to achieve the enhancement of steroid solubility at the same level,thus the negative effects of surfactants on mycelial growth were reduced and steroid conversion was therefore improved more.It was also shown that the surfactants and ultrasonic irradiation used jointly could result in better substrate dispersion and dissolution than surfactants used alone,and the best result was obtained when the comparatively high intensity (12.4 W/cm 2) and short time (5min) of ultrasonic irradiation were performed.
