RANDOM AMPLIFIED POLYMORPHIC DNA(RAPD)REVEALED THE HETEROKARYON OF AGARICUS ARVENSIS PRODUCED BY MATING REACTIONS
- VernacularTitle:随机扩增多态DNA(RAPD)在野蘑菇杂交育种中的应用
- Author:
Rong-Chun LI
;
Li-Mei ZHAN
;
- Publication Type:Journal Article
- Keywords:
RAPD,Agaricus arvensis, Mating reaction.
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
s: The horse mushroom (Agaricus arvensis Schaeff ex Fr.) is one of most excellent edible mushroom,and was cultivated in world at resent year.But up to now,the breeding systems and life-cycle of horse mushroom has been poorly know.In this study,the random amplified polymorphic DNA was employed to analyse the mating reaction between self-fertile homokaryonic isolates by paired.The results demonstrated: (1) the mating reaction happened and the heterokaryonic offspring formed when the intercompatible self-fertile homokaryonic isolates were paired;(2) perhaps the horse mushroom is possessed of double breeding systems-homothallism and heterothallism;(3) RAPD is very effective method for verifying artificially synthesized heterokaryons and monitoring the transmission of genetic loci in mating of fungi.
