RAPID PREPARATION OF PCR TEMPLATE OF SACCHAROMYCES CEREVISIAE PLASMID AND GENOMIC DNA
- VernacularTitle:快速制备酵母质粒和基因组DNA PCR模板
- Author:
Qiu-Yun LIU
;
Xi LUO
;
Kang-Ze HE
;
Bao-Jian LI
;
- Publication Type:Journal Article
- Keywords:
Boiling preparation,PCR template
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
A procedure for the preparation of PCR template from Saccharomyces cerevisiae using boiling method is described,and arg-13 gene from low copy ARSCEN plasmid and ymc1 gene from genomic DNA are amplified with high efficiency respectively.
