COLONIZATON OF STREPTOMYCIN RESISTANT RL-4 OF AS_(818) ON SOYBEAN RHIZOSPHERE
- VernacularTitle:生防菌AS_(818)抗药性标记株在大豆根际定殖
- Author:
Zan-Hua TONG
;
Rong-Jun GUO
;
- Publication Type:Journal Article
- Keywords:
Colonization,Soybean rhizosphere
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Spore suspension of Streptomyce s griseus AS 818 (10 6/mL)was radiated b y Co 60 -radiation,the radiation dosage was 1?10 4rad.And 5 streptomy cin resis tant mutants (above 50?g/mL) were screened,then the resistant level of mutant RL-4 was induced to 100?g/mL.The number of RL-4 colonizing on soybean roots pl anted on 1% Water agar or in sterile soil was determined.The results showed tha t it could colonize on soybean rhizosphere and rhizoplane for a short period,bu t there is a little difference.When it was examined in 1% Water agar,the numb er of RL-4 raised gradually in soybean rhizoplane in 4 weeks.But when examined in sterile soil,RL-4 reduced about 100 times at the 1 st week,then raised gradually and reached the highest point at the 3 rd week,and decreased aga in at the 4 th week,In the rhizoplane,it reduced gradually from the 1 st week,and couldn't be examined at the 4 th week. Mutant RL-4 couldn't be found in soybean endorhizosphere by tissue printing.
