Isolation,purification,physico-chemical properties and biological activities of a polysaccharide SPPC-1 from Spirulina Platensis
- VernacularTitle:钝顶螺旋藻多糖SPPC-1的分离纯化、性质及活性的研究
- Author:
Zhongfu WANG
;
Zhiying PENG
;
Linjuan HUANG
;
Gengyuan TIAN
;
- Publication Type:Journal Article
- Keywords:
Spirulina platensis;
Polysaccharide;
Biological activities
- From:
Chinese Journal of Marine Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
A polysaccharide SPPC 1 was isolated from spirulina platensis by acetone fractional precipation, free protein was removed by trichloroacetic acid(TCA). Purification of the polysaccharide by Sephadex G 75 colume and CM Sephadex C 50 colume.The homogeneity of this polysaccharide was proved by HPLC and CE. molecular weight was 1.62?10 6. GC showed that the glycoconjugate was composed of Rha,Xyl,Man and Glc. Molar ratio is Rha:Xyl:Man:Glc=6.24:0.37:0.13:0.71. IR, 1HNMR spectrum indicated the presence of ? linkage glycoside. ESR experiment showed that SPPC 1 could eliminate O . 2 radicals.
