Separation and purification of B-Phycoerythrin in Porphyridium cruentum
- VernacularTitle:紫球藻B-藻红蛋白的分离纯化
- Author:
Shaohong WEN
;
Chenglong ZHAO
;
Liping ZHANG
;
- Publication Type:Journal Article
- Keywords:
Porphyridium cruentum;
B Phycoerythrin;
purification
- From:
Chinese Journal of Marine Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The B Phycoerythrin(B PE) could both be separated after crude phycobiliproteins from Porphyridium cruentum were precipitated in ammonium sulphate and dialyzed,then chromatographed on a hydroxylapatite (HA) Column and a Sephadex G 100 column respectively.The purity of B PE separated by two methods was 4.92 and 3.78 (A 545 nm/A 280 nm) respectively.When the purified B PE was electrophoresed under polyacryiamide gradient gel electrophoresis condition,only one band was observed.The B PE possessed an absorption maximum at 545nm and a room fluoresence emission at 574.5nm.
