Comparison Researches on the Mode of Cell Death Induced by NDV-Strain Changchun and NDV-Strain Siping
- VernacularTitle:新城疫病毒长春株和四平株致细胞死亡方式的研究
- Author:
Wei GONG
;
Ningyi JIN
;
Lijuan XUE
;
Qinfang LUO
;
Dahui SUN
;
Tao GE
;
Ping LI
;
- Publication Type:Journal Article
- Keywords:
NDV;
apoptosis;
necrosis;
tumor cell
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the mode of cell death caused by NDV strain Changchun and NDV strain Siping. Methods: Plaque formation, cell suppression test, gel electrophoresis, and TUNEL assay were used after the cells were infected by NDV. Results: The apparently pathological changes were observed in chicken embryo fibroblasts, BHK, Hela, Hep 2, HCT and OS 732 tumor cells, but not in Wish cells. The higher suppressed effect on tumor cells was found in the NDV strain Changchun than that in the NDV strain Siping. There was no dose effect relationship between NDV and tumor cell suppression, only optimum dose NDV could cause maximal tumor cells inhibitory effect. Conclusion: The mode of cell death might be different after infection of NDV. The NDV strain Changchun killed tumor cells mainly through apoptosis, while the NDV strain Siping killed tumor cells mainly through necrosis. \[
