Strategy on Enhancement of bFGF Gene Expression and Purification of Its Products
- VernacularTitle:提高bFGF基因表达水平的策略及其表达产物的纯化工艺
- Author:
Lixin WANG
;
Yajuan LIU
;
Jian LIN
;
Hongxue FAN
;
Ningyi JIN
;
- Publication Type:Journal Article
- Keywords:
bFGF;
ATG;
SD sequence;
expression;
purification
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To adjust the distance between SD sequence and ATG in the same expressive plasmid pLX1 to enhance expression of heterologous bFGF gene in E. coli. Methods: Adjusting the different distance between SD sequence and ATG by Klenow and Mung Bean Nuclease. SDS PAGE and Western blot showed the expressed protein bFGF in E.coli. bFGF proteins were purified by HPHIC, HPGFC and HAC. Biological activity was examined by MTT. Results: Recombinant plasmids pLX2, pLX3 were obtained and the expressive levels were 8.03%, 9.9% respectively. Also the purified bFGF was obtained by HPHIC, HPGFC, HAC and its ED 50 was 2.29 ng/ml. Conclusion: Increasing the bFGF gene dosage by adjusting the distance between SD sequence and ATG could increase the expression level of a desired protein.
