PCR OF THE MAIN REPETITION SEQUENCE OF THE INAZ GENE IN THE DNA OF INA~- AND INA~+ BACTERIA AND FUNGI
- VernacularTitle:冰核微生物中冰核基因重复序列PCR分析
- Author:
Tingchang ZHAO
;
Fuzai SUN
;
Dazhi JIANG
;
Chaorong TANG
;
- Publication Type:Journal Article
- Keywords:
INA Organism,inaZ gene,PCR
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The oligonucleotide primer having the 5’\|GCCGGTTATGGC AGCACGCTGACC\|3’ was synthesized,which encodes a main repetition motif of protein produced by the inaZ gene.PCR of the primer in the DNA of INA - and INA + bacteria and fungi was done.All of the DNA of INA - and INA + bacteria and fungi expressed bands,the results show that the primer did not work for distinguishing the INA - and INA + organisms and for identification of the ice nucleation activity of the INA organisms by the size and brightness of the PCR products.
