THE ANTIBACTERIAL PEPTIDE CM4 INHIBITS REGENERATION OF SACCHAROMYCES CEREVISIAE PROTOPLAST BY DAMAGING ON THE PLASMA MEMBRANES
- VernacularTitle:抗菌肽CM4对酿酒酵母原生质体再生抑制作用的研究
- Author:
Jinshu XU
;
Shuangquan ZHANG
;
Yiaomei YAN
;
Ping LIU
;
Nan LIU
;
- Publication Type:Journal Article
- Keywords:
Antibacterial peptide CM4,Saccharomyces cerevisiae protoplasts,Ratio of regeneration,Confocal laser scanning microscopy
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The antibacterial peptide CM4 having potent antifungal activity on inhibitiong the cell wall regeneration of Saccharomyces cerevisiae protoplasts.When the peptide increased,the ratio of the regenerated colonies drop obviously.To study the antifungal mechanism of the antibacterial peptide,fluorescence\|labeled peptide mixted with the protoplast of yeast,then confocal laser scanning microscopy were performed.The results indicated that the peptides interactted with the protoplast membrane and damaged the structure of the membrane,then the permeation of protoplast changed.Finally the protoplasts with the peptide failed to regenerate the cell walls leading to killing the cell.
