REMOVING UNSPECIFIC ANTIBODIES WITH SEPHACRYL GEL COUPLING OF E COLI PROTEIN BY PERIODATE OXIDATION
- VernacularTitle:过碘酸盐氧化法制备固相载体除抗非目的蛋白抗体
- Author:
Xiangguo LIU
;
Gong YANG
;
Bingsheng QIU
;
- Publication Type:Journal Article
- Keywords:
Sephacryl S\|300,Antibodies,Western Blot
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The total proteins of plain E.coli(DH5 ?)were coupled to the Sephacryl S300 oxidized with periodate,and the preliminarily purified antiserum was loaded repeatedly on the column for 18 hours.The unspecific anti\|E.coli antibodies would be absorbed and the antibodies would be purified.It was more specific to do western blot using antibodies treated by this method than using ones without treatment.
