BIODEGRADABLE POLY (D,L-LACTIDE-CO-GLYCOLIC ACID) MICROSPHERES USED AS ADJUVANT IN DNA IMMUNIZATION OF HEPATITIS B VIRUS S GENE

VernacularTitle:生物可降解微球作为乙型肝炎基因免疫佐剂的研究
Author: Fei GONG ; Shu-De JIANG ; Wei-Dong LI ; Jun-Ying CHEN ;
Publication Type:Journal Article
Keywords: Poly(D,L-lactide-co-glycolic acid),DNA immunization, Hepatitis B virus S antigen (HbsAg)
From: Microbiology 1992;0(02):-
CountryChina
Language:Chinese
Abstract: Study the immunological adjuvant function of biodegradable microspheres for DNA immunization. Empty poly (D, L-lactide-co-glycolic acid) microspheres were prepared using the water- inoil-in-water (w-o-w) technique; A plasmid DNA pRc-CMV encoding hepatitis B virus S antigen was constructed; The mixture of the microspheres and the plasmid DNA was prepared by incubation method. The mixture was administered to Balb/c mice by intramuscular injection. Result: The high antibody titer(1:1600) of intramuscular injection of the mixture of microspheres and the plasmid DNA was obtained, similar to that of intramuscular injection of the mixture of AL(OH)3 and hepatitis B virus S antigen; while intramuscular injection the plasmid DNA elicited no serum antibody respones. Conclusion: biodegradable microspheres may be used as an good adjuvant for DNA immunization.