Screening the Mimic Antigen Epitopes of Triosephosphate Isomerase of Schistosoma japonicum Chinese Strain (Sjc Tpi) with Random Phage Peptide Library
- VernacularTitle:用噬菌体肽库筛选SjC-TPI的模拟抗原表位
- Author:
Chuanxin YU
;
Yinchang ZHU
;
Xuren YIN
;
Wei HE
;
Yongliang XU
;
Xiaohong GUAN
;
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum , triose phosphate isomerase(TPI), mimic antigen epitope
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the mimic antigen epitopes of the triose phosphate isomerase of Schistosoma japonicum Chinese strain (SjC TPI) and investigate their immunogenicity. Methods The random phage peptide library (PH\^D\^ 12) was screened with the purified antibody(IgG) against SjC TPI to get the positive phage which contained the mimic antigen epitopes of SjC TPI, and the immuno characterization of the mimic antigen epitopes were investigated. Results Two mimic antigen epitopes (M1, M2) of SjC TPI were obtained. The immuno sera of mice (Kunming strain) against the positive phages could recognize both the SjC TPI and the protein of the positive phages. The DNA sequencing data showed no homology between the sequences of the deduced amino acid of the two mimic antigen peptides and the amino acid of SjC TPI. Conclusion The two mimic antigen epitopes of SjC TPI obtained are imitative epitopes of the configuration antigen of SjC TPI.
