The establishment of a modified method to evaluate angiogenesis of rat rings in serum free medium
- VernacularTitle:一种无血清培养动脉环血管生成改良方法的建立
- Author:
Lichun WANG
;
Meiyu GENG
;
- Publication Type:Journal Article
- Keywords:
Angiogenesis;
Modified method;
Aorta rings;
Wistar rats;
MCDB131 medium
- From:
Chinese Journal of Marine Drugs
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The establishment of a modified method to evaluate angiogenesis of rat rings in serum-free medium was investigated in this paper. The aorta rings of Wistar rats were embedded in fibrinogen gel in 48 well plates, and cultured in an optimized serum free medium MCDB131. Results indicated that new capillaries spouted on 4th day, and entered into growth phase from 6th day, and peaked on 13th day and remained unchanged thereafter.