SELECTION OF THE ACID AND HEAT-RESISTANT PULLULANASE-PRODUCING STRAIN AND ITS FERMENTATION CONDITIONS
- VernacularTitle:耐酸耐热普鲁兰酶菌株的筛选及发酵条件的研究
- Author:
Baoying TANG
;
Xiaohui ZHU
;
Jia LIU
;
- Publication Type:Journal Article
- Keywords:
Pullalanase, Bacillus, Mutation, Enzyme formation and properties.
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
A strain producing pullulanase was isolated from soil and mutated by UV and EMS with the increase of pullulanase activity from 1.6 to 5.4 u/mL. The fermentation conditions and medium were studied and optimized. The enzyme activity can reach 8.8 u/mL under the optimal fermentation conditions and medium. The optimal pH and temperature for enzyme are 4.6 and 75℃ respectively. It shows a high stabiliby during pH4.0～8.0 at 55℃.
