In vitro Inhibitory Effect on Growth of Human Glioma Cells with p21 Gene Mediated by a Novel Gene Delivery System
- VernacularTitle:新型基因转移系统介导p21基因治疗人脑胶质瘤的体外研究
- Author:
Xiang LIU
;
Junsong HAN
;
Peikun TIAN
;
Jianren GU
;
- Publication Type:Journal Article
- Keywords:
EGF-Receptor;
glioma;
targeted non-viral vector;
p21WAF-1;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of novel targeted non-viral vector in gene therapy of human glioma. Methods: The EGF-R targeting gene delivery system GE7 was constructed. Human Glioma cell line U251 was transfected in vitro with ?－gal as reportor gene and p21 as therapeutic gene using this gene delivery system. By means of the assay of ?-galactosidase staining, Western blotting, in situ end labeling apoptosis cells and DNA ladder, the transferring of exogenous genes and the apoptosis of the tumor cells were examined.Results: It was showed that gene transfer efficiency is over 80%. When transfected with p21 gene, the growth of cells was inhibited significantly, and the apoptosis was detected in the transfected cell by the methods of in situ end labeling and DNA ladder. Conclusion: The GE7 gene delivery system has the ability to transfer exogenous gene to tumor cells and the expression of the therapeutic gene can inhibit the growth of the cells.
