Relationship Between Different c-myc Expression in Differentiation or Proliferative Suppression of HL-60 Cells
- VernacularTitle:c-myc基因表达与白血病细胞分化诱导及增殖受抑的相关性
- Author:
Yaochuan YUAN
;
Suyun SHEN
;
- Publication Type:Journal Article
- Keywords:
recombinant human tumor necrosis factor - a;
oncogene;
expression;
leukemia;
differentiation;
proliferation
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
We compared the changes of c-myc expression, cell growth kinetics, ability to reduce NBT and 3H -TdR incorporation rate of HL-60 cells in low concentration of FCS, or treated by TNF or no treatment, respectively. The results showed that, although the prolifcrative suppression of HL-60 cells treated by 50U/ml TNF was mimicked by adding 1. 0%and 0. 5%FCS in culture media, the differentiation had notappeared and it had not effected on c - myc expression, either. TNF (50U/ml) could inhibit proliferation of HL-60 cells during inducing differentiation and the expression of c-myc oncogene decreased remarkedly. The results indicated that decreased expression of c-myc in TNF treated HL-60 cells were mainly connected with differentiate maturation but not with proliferative suppression.
