IMPROVEMENT OF MOLECULAR CLONING RECIPIENT IN BACILLUS SUBTILIS
- VernacularTitle:枯草芽孢杆菌分子克隆系统受体的改造
- Author:
Xinghua GUO
;
Shifang JIA
;
Yi XU
;
- Publication Type:Journal Article
- Keywords:
Bacillus subtilis;
stable recipient for plasmid
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The results of experiment showed that B. subtilis BR151 and MI112 containing plasmid pUB110 in LB liquid medium is more stabl than in LB slant. Stable recipient strain BSG for plasmid pUB110 was obtained by mutagenesis with NTG. The strain BSG is more efficient recipient of transformation for shuttle vectors pPTE4 and pBE2 from E.coli than its parent. The chimeric plasmids which contain small inserts are stable in strain BSG after 30 generations.
