STUDY ON HERPES SIMPLEX VIRUS TYPING WITH MOLECULAR BIOLOGICAL METHODS
- VernacularTitle:单纯疱疹病毒分子生物学分型方法的研究
- Author:
Qiang WU
;
Yonglie CHU
;
Yilan FANG
;
- Publication Type:Journal Article
- Keywords:
Herpes Simplex Virus;
DNA probe;
McAb;
Typing
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
A total of twenty eight HSV isolates from the patients were typed by using HSV typespecific monoclonal antibodies (McAb). Comparison of typing results with McAb in three Sero-lminunological methods with molecular hybridization indicated 100% concordance in the results. But the typing rates were quite different among the various methods (ELISA 64%, IFA and EIA 82%, Hybridization 100%). The results demonstrate that the molecular hybrydization with HSV type-specific probes was highly sensitive and specific, and the method of EIA with HSV type-specific McAb was accurate, cheap, rapid and practical.
