COLONY HYBRIDIZATION WITH BIOTIN LABELED DNA PROBE
- VernacularTitle:用生物素标记DNA探针进行菌落原位杂交
- Author:
Sanliang JIANG
;
Sizhong ZHANG
;
- Publication Type:Journal Article
- Keywords:
Biotin labeled DNA probe;
Colony hybridization;
Colony screening
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
A simple, rapid method for colony hybridization has been developed. The DNA probes were labeled by digo xigenin. The signal of hybridization was detected by streptavidin and poly (AP) system. The results showed that this method is sensitive, specific and repoducible, it can be used for colony hybridization instead of isotopic.
