THE TECHNOLOGICAL CONDITIONS OF PRODUCING 2-KETO-LGULONIC ACID FROM D-GLUCOSE BY DIRECT FERMENTATION
- VernacularTitle:D-葡萄糖串联发酵生产2-酮基-L-古龙酸的工艺条件
- Author:
Gang ZHANG
;
Xiande LAN
;
Xiaobin FAN
;
Zhihua HAN
;
Guanglin YIN
;
Zhifang MA
;
Wenling DONG
;
- Publication Type:Journal Article
- Keywords:
2-Keto-L-gulonic acid;
Fermentation;
Vitamin C
- From:
Microbiology
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
2-Keto-L-gulonic acid (2-KLG), the precurcor of L-ascorbic acid synthesis, was prepared directly from D-glucose by tandem fermentation. In the first step fermentation Erwinia sp. SCB 247 translated D-glucose to 2,5-diketo-D-gluconate (2,5-DKG), which accumenlated 180 mg 2,5-DKG per ml in the broth. In the second step fermentation Corynebacterium sp. SCB 3058 reduced 2,5-DKG to 2-KLG, which accumulated 35 mg 2-KLG per ml in the broth. This reductive fermentation was obtained under aerobic conditions by adding a hydrogen donor such as glucose.The average yield of five batches fermentation was 56.3 mol%, from D-glucose to 2-KLG in 10 L fermentor.
