Content Determination of Astragaloside Ⅳ in Qingshen Jianfei Tablets by HPLC-ELSD
- VernacularTitle:HPLC-ELSD法测定轻身减肥片中黄芪甲苷的含量
- Author:
Zhan LI
;
Mei-Rong LI
;
Guo-Qiong LUO
;
Qun RUAN
;
- Publication Type:Journal Article
- Keywords:
Qingshen Jianfei Tablets;
astragaloside Ⅳ;
HPLC-ELSD;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC-ELSD method for determining the content of astragaloside Ⅳ in Qingshen Jianfei Tablets. Methods Stationary phase was C_(18) column (4.6 mm?250 mm, 5 ?m), mobile phase was acetonitrice-water (35 : 65). The evaporation temperature was 120 ℃, the pulverization temperatire was 80 ℃, the flow rate was 1.6 mL/min and column temperature was 35 ℃. Results The standard curve was linear within the range of 1.03～8.24 ?g, r=0.9999. The average recovery was 99.35% with RSD = 1.36% (n = 6). Conclusion The established method is simple and accurate, with good reproducibility and high precision, and suitable for the determination of astragaloside Ⅳ in Qingshen Jianfei Tablets.
