Effect of Xiaoyu Huatan Decoction on Hepatic Apoptosis and Expression of Apoptosis-related Gene in Rats with Nonalcoholic Fatty Liver Disease
- VernacularTitle:消瘀化痰饮对非酒精性脂肪肝大鼠肝细胞凋亡及相关调控基因的影响
- Author:
Yixin ZHANG
;
Hui MIAO
;
Cuiping WEI
;
Hua ZHOU
;
Zhai WU
;
Chang LI
;
- Publication Type:Journal Article
- Keywords:
Xiaoyu Huatan Decoction;
Nonalcoholic fatty liver;
Apoptosis;
Bcl-2;
Bax
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Xiaoyu Huatan Decoction (XHD) on hepatic apoptosis and apoptosis- related gene Bcl-2,Bax expression in nonalcoholic fatty liver disease(NAFLD)rats,and to explore its therapeutic ef- fect for NAFLD.Methods The rat model of NAFLD was induced by feeding high fat forage.Dongbao Gantai was used as the positive control drug.Flow cytometry(FCM)was used to detect the hepatic apoptosis and expression of Bcl-2 and Bax,and optical microscope was used to observe the degree of hepatic steatosis.Results Moderate or serious steatosis of liver cells was tound under optical microscope,and there existed a few hepatic necrosis.The apoptotic rate and FI expres- sion of Bax in the model group were significantly increased (P
