Anti-oxidation Effects of Compound Chinese Medicine of Yiqiling on Hypertensive Rats
- VernacularTitle:益气苓对自发性高血压大鼠抗氧化作用的研究
- Author:
Ying ZHANG
;
Weiguo QIANG
;
- Publication Type:Journal Article
- Keywords:
Yiqiling;
Spontaneous|yhypertensiverats;
SOD;
MDA;
8-isoPGF2?;
cDNAmicroarray
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti-oxidation effects of compound Chinese medicine of Yiqiling,on the spon- taneously hypertensive rats(SHR) and to explore the new target for improving the prognosis of hypertension.Method SHR were randomly divided into 2 groups:Yiqiling group and the control group.The serum content of malondiadehyde (MDA) and superoxide dismutase(SOD),and the urine content of 8-iso prostaglandin F2?(8-isoPGF2?) in two groups were measured.Examination of the gene expression profile was analyzed by using cDNA microarray.Three of the anti-oxidation genes were selected to undergo RT-PCR and quantitative real-time PCR.Results The 8-isoPGF2?urine level and MDA serum level were lower,and SOD serum level was higher in Yiqiling group than those in the control group,the differences of the above indexes had statistical significance.In Yiqiling group the expression of 354 genes was up-regulated and that of 121 genes was down-regulated in comparison with the control group.The results of the Hsphl,Hspca and Pam genes detected by RT-PCR and quantitative real-time PCR were consistent with those detected by the microarray examination.Conclusion Yiqiling has anti-oxidation effects and improvement on the prognosis of SHR,and its possible mechanism is related with the regulation of Hsphl,Hspca and Pam genes expression.
