Effects of Psoralen and Ultraviolet Chemical Therapy on Cell Apoptosis and FasL Expression in Human Leukemia Cells
- VernacularTitle:补骨脂素光化学疗法对人白血病细胞凋亡及FasL表达的影响
- Author:
Nannan CHEN
;
Shilin HUANG
;
Yang XIANG
;
Li ZHANG
;
Dejie ZHANG
;
Yubin CHENG
;
- Publication Type:Journal Article
- Keywords:
PUVA therapy;
Leukemia;
HL-60 cells;
K562 cells;
NB4 cells;
Apoptosis;
FasL
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of psoralen(PSO)and long-wave ultraviolet chemical therapy(PUVA) on cell apoptosis and expressions of FasL in HL-60,K562,and NB4 leukemia cells.Methods The cells were taken as the studying objects and their apoptosis ratios,ultrastructure changes and the expressions of FasL were detected in or- der to observe the effects of PSO extracted from Chinese medicine and ultraviolet at 360 nm on human leukemia cells.The factor design and analysis of variance were used to analyze the interaction among the factors.Results(1)PSO,UVA and PUVA all induced the apoptosis and the effects of PUVA were stronger than the other two.(2)Obvious changes of ultra- structure of leukemia cells were found under electron microscope after treatment with PUVA.(3)PSO,UVA and PUVA down-regulated FasL gene and protein expression levels,and the effects of PUVA are the strongest.Conclusion PUVA can induce the apoptosis of human leukemia cells and its effects are the strongest.One of the pathway of PUVA to induce apoptosis is by down-regulating the expression of FasL gene.
