Study on Loganin and Morronside Concentrations in Rat Serum Containing Liuwei Dihuang Formula by SPE-HPLC and the Variety of Different Combinations
- VernacularTitle:固相萃取HPLC法研究六昧地黄方中马钱素和莫罗苷的血药浓度及配伍变化
- Author:
Lingling JU
;
Hongmei WEN
;
Xin MA
;
Huimin BIAN
;
- Publication Type:Journal Article
- Keywords:
Liuwei Dihuang formula;
Loganin;
Morroniside;
Serum concentration
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a sensitive and specific liquid chromatography method for the determination of loganin and morroniside in rat serum containing Liuwei Dihuang Formula(LDF).Methods Loganin and morroniside were extracted from the rat serum using SPE column,then separated on a C_(18)column.A HPLC method for determining serum concentration of mor- roniside and loganin in rats before and after administration LDF was established.Results A good linear calibration curve of lo- ganin and morroniside was obtained.The recovery of the method was in the range of 95 %～105 %.The extracted recovery was more than 75 % ,and within-day and intra-day precisions were less than 12 %.Conclusion This method is sensitive, simple and rapid for monitoring of drug level in the study on pharmacokinetics and different combination of LDF.
