HPLC Determination of Contents of Four Flavonoids in Different Samples of Sophora flavescens Ait.in the Market
- VernacularTitle:HPLC测定不同地区苦参市售饮片中四种黄酮类成分的含量
- Author:
Chao XU
;
Lijun ZHU
;
Bin LIU
;
Renbing SHI
;
- Publication Type:Journal Article
- Keywords:
Sophoraflavescens Ait.;
Trifolirhizin;
Nor-kurarinone;
Xanthonumal;
Kuraridin;
Determination;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the contents of four fiavonoids(trifolirhizin,nor-kurarinone,xanthonumal and kuraridin)in 25 samples of Sophora flavescens Air.in the market.Methods The determination was carried out on Phe- nomenex Luna C_(18)column(250 mm?4.6 mm,5?m),using Acetonitrile-H_2O as mobile phase,at a flow rate of 1.0 mL/min and detected at the wavelength of 310 nm for trifolirhizin,294 nm for nor-kurarinone and,365 nm for xan- thonumal and kuraridin,and column temperature at 35℃.Results The contents of 4 flavonoids in 25 samples of Sophora flavescens Air.in market were in the ranges of 0.05 %～0.32 % for trifolirhizin,0.05 %～0.51% for nor- kurarinone,0.55?10~(-3)%～4.87?10~(-3)% for xanthonumal and 0.06 %～0.23 % for kuraridin.Conclusion The contents of 4 flavonoids in the samples from the market vary notably,and this should be taken into the quality control of Sophora flavescens Ait.
