Determination of Paeoniflorin in Cervipower Capsules by RP-HPLC
- VernacularTitle:RP-HPLC法测定羓鸡丸胶囊中芍药苷的含量
- Author:
Qiwen LU
;
Jian LI
;
Yanting XIE
;
Peng XIAO
;
- Publication Type:Journal Article
- Keywords:
Cervipower Capsules;
Paeoniflorin;
RP-HPLC;
Assay
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the RP-HPLC method for the determination of paeoniflorin in Cervipower Capsules. Methods Diamonsil~(TM)C_(18)column was used,with a mobile phase of A(methanol)-B(0.025 mol?L~(-1)disodium hydro- gen phosphate,adjusting pH being 7.4 with 0.025 mol?L~(-1)potassium dihydrogen phosphate),and with a gradient elu- tion,the flow rate was 1.0 mL?min~(-1),the detection wavelength was 230 nm,and the column temperature was 30℃. Result The linear range was 15.10～105.70?g?mL~(-1),r=0.999 9,and the average recovery for paeoniflorin was 100.1%,with RSD 1.94 %(n=9).Conclusion The method is accurate and reproducible,and can be used to deter- mine the content of paeoniflorin in Cervipower Capsules.
