Relationship Between IgE-Mediated Basophil Histamine Releasability and Airway Hyperresponsiveness or Atopy Markers in Atopic Children.
- Author:
Soo Jong HONG
1
;
Jin Kyong CHUN
;
Bong Seong KIM
;
Jeong Yeon SHIM
;
Sang Heon CHO
;
Kyung Up MIN
Author Information
1. Department of Pediatrics, Asan Medical Center, Korea.
- Publication Type:Original Article
- Keywords:
IgE-mediated basophil histamine releasability;
Airway hyperresponsiveness;
Atopy;
Immunoglobulin E;
D.p-specific IgE antibody
- MeSH:
Asthma;
Basophils*;
Child*;
Diagnosis;
Eosinophils;
Histamine Release;
Histamine*;
Humans;
Immunoglobulin E;
Methacholine Chloride;
Respiratory Function Tests
- From:Pediatric Allergy and Respiratory Disease
2000;10(4):299-307
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Our previous data suggested that IgE-mediated histamine release from basophil was elevated in the atopic asthmatic children. Basophil may play an important role in the development of the IgE-dependent, late phase response in allergen induced airway disease. So the existence of enhanced basophil histamine release in asthma could promote airway reactivity and obstruction. PURPOSE: The purpose of this study is to determine the relationship between IgE-mediated basophil histamine releasability (BHR) and airway hyperresponsiveness or markers of atopy in atopic children. METHODS: Twelve atopic asthmatics and four healthy atopics who were sensitive to D.p and D.f were selected. Their median age was 11.2 years old, their mean serum IgE level was 897+/-276 IU/mL and mean total eosinophil count was 536+/-71/mm3. Total eosinophil counts, total IgE, D.p and D.f-specific IgE, pulmonary function test, and methacholine provocation test were performed. IgE-mediated basophil histamine release by D.f allergen and goat-antihuman IgE antibody were measured by automated fluorometric assay. The relationship between histamine release and airway hyperresponsiveness or atopic markers was investigated. RESULTS: PC20 inversely correlated with anti-IgE antibody-mediated BHR (r=-0.50, P<0.05). Serum total IgE concentration correlated with anti-IgE antibody-mediated BHR (r=0.54, P<0.05). Serum concentrations of specific IgE to D.p correlated with anti-IgE antibody-mediated BHR (r=0.66, P<0.05). PC20 correlated correlated with FEF25-75% (r=0.75, P<0.05) and inversely with the total eosinophil counts (r=-0.69, P< 0.01). CONCLUSION: IgE-mediated basophil histamine releasability is inversely correlated with airway hyperresponsiveness, and correlated with total or specific-IgE in atopic children. These findings suggest that basophil histamine releasability is easy and useful method of diagnosis and monitoring response to treatment in atopic disease.
