In-vitro Observation of ?-asarone for Counteracting Arteriosclerosis
- VernacularTitle:?-细辛醚抗动脉硬化的体外作用观察
- Author:
Yuping HE
;
Yongqi FANG
;
Yong JIANG
;
Yi LIANG
;
Shuying WANG
;
- Publication Type:Journal Article
- Keywords:
ARTERIOSCLEROSIS/TCD therapy;
?-ASARONE/pharmacology;
APOPTOSIS;
CELL CULTURE
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protective effect of?-asarone,the active components from Rhizoma Acori Tarinowii,on human umbilical venous endothelial cell strain ECV304 injury induced by oxidized low-density lipoprotein(ox-LDL)and to investigate its influence on proliferation of vascular smooth muscle cells(VSMC). Methods The well-grown ECV304 cells were randomized into 5 groups:normal group,control group,high-, middle-and low-dose?-asarone groups.Except the normal group,ECV in the other groups were co-cultured with ox-LDL 25?g/mL,and the?-asarone groups were additionally with?-asarone 30,15 and 7.5?g/mL added respectively.Flow cytometry was used to detect the cell apoptotic rate and the mitochondrial membrane potential (MMP)of ECV304 as well as the proliferation index and the DNA content in different cell phases of VSMC. Results In the model group,the apoptotic rate of ECV was increased,MMP was decreased,and VSMC proliferation was promoted(P
