Effect of puerain on Apelin/APJ system in right ventricle of pulmonary hypertensive rats induced by hypoxia-hypercapnia
- VernacularTitle:葛根素对低氧高二氧化碳性肺动脉高压大鼠右心室Apelin/APJ系统的影响
- Author:
Ping HUANG
;
Yong-Sheng GONG
;
Xiao-Fang FAN
;
Qin-Hui ZHANG
;
Sun-Zhong MAO
;
Liang-Gang HU
;
- Publication Type:Journal Article
- Keywords:
puerain;
Apelin/APJ;
right ventricle hypertrophy;
pulmonary hypertension
- From:
Chinese Traditional and Herbal Drugs
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether the effect of puerarin on right ventricle hypertrophy of pulmonary hypertensive rats induced by chronic hypoxia-hypercapnia was related to new peptide Apelin or its receptor(APJ).Methods Thirty male Sprague-Dawley rats were randomly divided into three groups, they are control group,hypoxia-hypercapnia 4-week model group,and hypoxia-hypercapnia 4-week plus puerarin group.The concentrations of Apelin-36 protein in plasma and homogenate of right ventricular muscle were detected by radioimmunoassay.The mRNA expressions of Apelin and APJ in right ventricu- lar muscle were measured by semi-quantitive reverse transcription polymerase chain reaction(RT-PCR). Results The weight ratio of right ventricle to left ventricle plus septum[RV/(LV+S)] in model group was significantly higher than that in control group(P0.05).The plasma concen- tration of Apelin-36 protein in model group was significantly higher than that in control group(P
