Study of a serum protein fingerprint diagnostic model in patients with hypertensive disorder complicating pregnancy
- VernacularTitle:血清蛋白指纹图谱用于建立妊娠期高血压疾病诊断模型的研究
- Author:
Li-Yun GONG
;
Zhen-Yu ZHANG
;
Yan-Hua ZHENG
;
Jian-Zhong ZHANG
;
- Publication Type:Journal Article
- Keywords:
Pregnancy complications;
cardiovascular;
Hypertension;
Blood proteins;
Peptide mapping;
Proteome
- From:
Chinese Journal of Obstetrics and Gynecology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the alterations of serum protein fingerprint in patients with hypertensive disorder complicating pregnancy(HDCP),screen serum biomarker and establish a diagnostic model of HDCP.Methods Surface-enhanced laser desorption lionization-time of flight-mass spectrometry (SELDI-TOF-MS)technology was used to analyze serum including 25 cases of HDCP patients and 30 cases of age-,gravity-and parity-matched healthy term pregnant women on IMAC3-Cu proteinchip before delivery. Biomarker Wizard and Biomarker Pattern software was used to detect protein peaks significantly different between HDCP and controls,and establish a primary diagnostic model of HDCP.This model was further evaluated by blind test using other 16 parts of serum protein fingerprint.Results Ten protein peaks were significantly different at the molecular range of 2000-50 000(P
