Changes of the glutamate transporter EAAC1mRNA expression and injuried nerve cell apoptosis in rat with cerebral ischemia/reperfusion injury treated by mild hypothermia
- VernacularTitle:亚低温处理大鼠脑缺血再灌注损伤后谷氨酸载体EAAC1mRNA表达和损伤神经细胞凋亡的变化
- Author:
Guo-Xiang WU
;
Cheng-Yan LI
;
Chun-Ying LIU
;
Xiao-Li ZOU
;
Jun XIA
;
- Publication Type:Journal Article
- Keywords:
Mild hypothermia;
Cerebral ischemia/reperfusion injury;
EAAC1mRNA;
Apoptosis
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes in the glutamate transporter EEAC1 mRNA expression and the injured nerve cell apoptosis after rat cerebral ischemia/reperfusion injury is treated with mild hypothermia. Methods The middle cerebral arteries(MCA)of Sprague-Dawley rats were occluded for 30 minutes,and reper- fused for 90 minutes.Using DIG-labeled CRNA probe and TUNEL,the positive rate of glutamate transporter EE- AC1 mRNA expression and apoptotic cell rate were determined in the sham-operated group,the control group and the mild hypothermia group,respectively.Results The positive rate of EEAC1 mRNA expression and apoptotic cell rate were significantly lower in sham-operated group than those in the control group(P
