Expression of Toll-like receptor 2 and 4 on colons of experimental colitis rats and the therapeutic effects of probiotics
- VernacularTitle:实验性结肠炎大鼠Toll样受体2和4表达及益生菌治疗的作用
- Author:
Wei LIU
;
Lan RONG
;
Wei-Qun DING
;
Yibin JIANG
;
Liang ZHONG
;
Dayu SUN
;
- Publication Type:Journal Article
- Keywords:
Colitis;
Toll-like receptor 2;
Toll-like receptor 1;
Probiotics;
Disease models,animal
- From:
Chinese Journal of Digestion
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protein and mRNA expressions of Toll-like receptor 2 (TLR2) and Toll-like receptor 4 (TLR4) on colons of rats with trinitrobenzene sulfonic acid (TNBS)- induced colitis,and to evaluate the effects of probiotics.Methods Thirty male Wistar rats were randomly divided into normal control group (NC group),model control group(UC group) and probiotics-treated group(PC group).The experimental colitis was induced by TNBS/ethanol enema.Rats in PC group were fed with Bifico [live probiotics of combined hifidobacterium(Bif),lactobaeillus(Lac) and enteroeoccus] by 2.2?10~9 CFU/d for 4 weeks.Inflammatory scores were studied.Expressions of protein and mRNA of TLR2 and TLR4 were measured by Western blot and real-time quantitative polymerase chain reaction (PCR),respectively.Results Inflammatory scores in NC group,UC group and PC group were 4.35?0.88,10.25?1.36 and 7.94?0.85,respectively.The inflammatory scores in PC group were decreased compared with that in UC group (P
