Improvement on isolation and culture of human endometrial glandular epithelial cells and stromal cells in vitro
- VernacularTitle:分离培养子宫内膜腺上皮和间质细胞方法的改进
- Author:
Jia WANG
;
Zhenwei YAO
;
Liangdan TANG
;
Liang ZHONG
;
Hua LINGHU
;
Jian QIAO
;
Beizhong LIU
;
- Publication Type:Journal Article
- Keywords:
Endometrium;
Glandular epithelial cells;
Stromal cells;
Cell culture
- From:
Journal of Chongqing Medical University
2007;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the isolation and culture of a better yield of purified human endometrial glandular epithelial cells and stromal cells. Methods:Digestion with high concentrated collagenase and DNase,filtratinn and adhesion purification were used to isolate,purify human endometrial glandular epithelial cells and stromal cells in 30samples. And the two kinds of cells were reproduced. Results:The endometrial cell culture was successfully established in 29 of 30 endometrium samples. From one gramme of endometrial tissue,the yields of glandular epithelial cells and stromal cells were (40～50)?10~6 and (40～50)?10~6 respectively. The purities of glandular epithelial cells and stromal cells were 96% and 98% respectively. Stromal cells could be reproduced into several passage. However,glandular epithelial cells could not be reproduced. Conclusion:A better yield of purified human endometrial glandular epithelial cells and stromal cells can be obtained by the modified culture procedure.
