Clinical study of the correlation among the levels of human corticotropin-releasing hormone, c-Fos and connexin-43 at onset of labor
- VernacularTitle:促肾上腺皮质激素释放激素、c-Fos及间隙连接蛋白43与分娩发动的相关性
- Author:
Xiang WU
;
Yi-Ling DING
;
Ling YU
;
- Publication Type:Journal Article
- Keywords:
Labor;
obstetric;
Corticotropin-releasing hormone;
Proto-oncogene proteins c-Fos;
Connexin 43
- From:
Chinese Journal of Obstetrics and Gynecology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the variation of corticotropin-releasing hormone(CRH)in plasma,the mRNA and protein expression of c-Fos and gap junction gene connexin-43(Cx43)in the myometrium of term pregnancy women,and to study the correlations among CRH,Cx43 and c-Fos at onset of labor.Methods 30 cases in labor(L group),30 cases not in labor(NL group)but in term pregnancy, and 30 cases of non-pregnant(NP group)women undergoing hysterectomy due to cervical intraepithelial neoplasia were recruited into this clinical study.Radioimmunoassay was employed to measure the concentration of CRH in plasma;in situ hybridization assay and streptravidin-biotin peroxidase(SP) immunohistoehemical techniques were respectively used to detect the expression levels of c-Fos mRNA,Cx43 mRNA and the corresponding proteins.Results(1)The concentration of CRH in L group(81.8?11.9) pmol/L was significantly higher than that in NL group(34.5?18.6)pmol/L(P
