Effects of bererine on basolateral potassium current of rat colonic crypt cells
- VernacularTitle:黄连素对大鼠结肠上皮隐窝细胞基底膜钾通道的影响
- Author:
Wan-Ling YIN
;
He-Sheng LUO
;
Jia CAI
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
Berberine;
Colonic;
Patch-clamp techniques;
Calium activated potassium current;
cAMP-activated potassium current
- From:
Chinese Journal of Digestion
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of berberine on basolateral calcium-activated potassium current I_K(Ca)and cAMP-activated potassium current I_K(cAMP)and its mechanism in treatment of secretory diarrhea.Methods The intact colonic crypt cells were isolated with EDTA solution.The effects of berberine(50,100,500?mol/L)on I_K(Ca)and I_K(cAMP)were detected by patch clamp technique under the conventional whole cell patch clamp mode.The solution of PSS was served as control.Results Berberine could significantly inhibite I_K(Ca)and I_K(cAMP)of rat colonic crypt cells(both P
