The co-stimulatory effect of growth factors on chondrogenesis induction of rat mesenchymai stem cells
- VernacularTitle:生长因子联合诱导大鼠间充质干细胞向软骨分化的实验研究
- Author:
Feng-Ying GAN
;
Chen TANG
;
De-Fu YE
;
Di-Bin GUO
;
- Publication Type:Journal Article
- Keywords:
Mesenehymal stem cells;
Growth substances;
Chondrogenesis;
Transforming growth factor-?_1;
Insulin-like growth factor-Ⅰ;
Bone morphogenetie protein-2
- From:
Chinese Journal of Rheumatology
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The present study aims to explore the optimal combination of growth factors, including TGF-?_1,IGF-I and BMP-2,for the induction of ehondrogenesis of MSCs in vitro monolayer culture. Methods MSCs were induced to differentiate into ehondrocytes in the medium with the following growth fac- tors:TGF-?_1,BMP-2 and IGF-I,either alone or in combination.We detected two cartilage specific markers in differentiated cells.Alcian blue stain was used for aggreean and RT-PCR for collagen type II mRNA.Results The level of Collagen typeⅡmRNA of group BMP-2+IGF-I,TGF-?_1,TGF-?_1+IGF-I,TGF-?_1+BMP-2 and TGF-?_1+IGF-I+BMP-2 was 0.147?0.062,0.478?0.069,0.489?0.022,0.653?0.013,0.807?0.051 respectively. Alcian blue stain in all gruups were positive.Conclusion The TGF-?_1+IGF-I+BMP-2 group is the most ef- fective combination in chondrogenesis induction.
