Nephritic changes of transgenic mice with high serum titer of anti-keratin autoantibody
- VernacularTitle:高滴度天然抗角蛋白自身抗体转基因小鼠的肾脏损害分析
- Author:
Li-Qiang ZHENG
;
Wei LI
;
Meng FU
;
Ying XING
;
Lei WANG
;
Wei-Jie GU
;
Yu-Feng LIU
;
- Publication Type:Journal Article
- Keywords:
Autoantibodies;
Mice,transgenic;
Kidney disease
- From:
Chinese Journal of Rheumatology
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the autoimmune status of kidney in transgenic mice with high serum titer of anti-keratin autoantibody(AK auto Ab).Methods AK auto-Ab transgenie mice were set-up with conventional procedures,and the mice older than 20 weeks were selected for study.Urinary protein was quan- tified by Bradford method,and SDS-PAGE electrophoresis was used to analyze the type of proteinuria.Speci- mens of kidney were subjected to HE and PAS stain and immunofluorescence analysis and electrum microscopy observation were also performed.Results Compared with littcrmates,transgenie mice showed higher urinary proteins level(P
