Effects of?1 adrenoceptor antisense on blood pressure and?1 adrenoceptor mRNA and protein in renal hypertension rats
- VernacularTitle:?1受体反义基因对肾性高血压大鼠心脏?受体的影响
- Author:
Yuan-Hong HANG
;
Shu-Guang LIN
;
Yan ZHOU
;
Jin-Ming WANG
;
Fang WANG
;
Xi-Yong YU
;
- Publication Type:Journal Article
- Keywords:
?1 receptor;
Two kidney 1 clip hypertension;
Antisense oligodeoxynucleoside
- From:
Chinese Journal of Emergency Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of 131 adrenoceptor anfisense on blood pressure and?1 adrenoceptor mRNA and protein levels in 2 kidney 1 clip(2K1C)rats.Method 2KIC hypertensive rots were produced by clipping renal artery of SD rats.Liposome/AS-ODNs 2.0 were tested intravenously in rats with 2KIC hypertension.Animals were divided into 5 groups(n=18 in each group):?1-AS-ODN group,?1-IN-ODN group,2K1C group,Sham group and SD group.Blood pressure was measured by tail-cuff method,the levels of myocardial?adreneceptor mRNA and protein were tested by RT-PCR and binding assay.Results On the basis of the magnitude and duration of hypotension,?1-AS-ODN decreased blood pressure by 39 mmHg at the most for 4 weeks.Compared with the 2KIC group,?1-AS-ODN did not significantly change the levels of myocardial?1 adrenoceptor mRNA but significantly decreased the levels of myocardial?1 adrenoceptor protein at 2,7,30 days (P
